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Isoelectric focusing
Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by differences in their isoelectric point (pI).[1][2] It is a type of zone electrophoresis usually performed on proteins in a gel that takes advantage of the fact that overall charge on the molecule of interest is a function of the pH of its surroundings.[3]
- ^ Bjellqvist, Bengt; Ek, Kristina; Righetti, Pier Giorgio; Gianazza, Elisabetta; Görg, Angelika; Westermeier, Reiner; Postel, Wilhelm (1982). "Isoelectric focusing in immobilized pH gradients: Principle, methodology and some applications". Journal of Biochemical and Biophysical Methods. 6 (4): 317–339. doi:10.1016/0165-022X(82)90013-6. ISSN 0165-022X. PMID 7142660.
- ^ Pier Giorgio Righetti (1 April 2000). Isoelectric Focusing: Theory, Methodology and Application. Elsevier. ISBN 978-0-08-085880-7.
- ^ David Edward Garfin (1990). "Isoelectric focusing". Guide to Protein Purification. Methods in Enzymology. Vol. 182. pp. 459–77. doi:10.1016/0076-6879(90)82037-3. ISBN 9780121820831. PMID 2314254.
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